Microbiology 151 (2005), 209-218; DOI 10.1099/mic.0.27265-0
Microbiology 151 (2005), 209-218; DOI 10.1099/mic.0.27265-0
© 2005 Society for General Microbiology
The formation of cyclopropane fatty acids in Salmonella enterica serovar Typhimurium
Bae Hoon Kim1,
Seungki Kim1,2,
Hyeon Guk Kim1,
Jin Lee1,
In Soo Lee3 and
Yong Keun Park1
1 Laboratory of Microbial Genetics, School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea
2 Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technology, Seoul 136-791, Korea
3 Department of Microbiology, Hannam University, DaeJeon 300-791, Korea
Correspondence
Yong Keun Park
ykpark{at}korea.ac.kr
The formation of cyclopropane fatty acid (CFA) and its role in the acid shock response in Salmonella enterica serovar Typhimurium (S. typhimurium) was investigated. Data obtained by GC/MS demonstrated that the CFA level in S. typhimurium increased upon its entry to the stationary phase, as in other bacteria. The cfa gene encoding CFA synthase was cloned, and mutants of the cfa gene were constructed by allelic exchange. A cfa mutant could not produce CFA and was sensitive to low pH. Introduction of a functional cfa gene into a cfa mutant cell made the mutant convert all unsaturated fatty acids to CFAs and partially restored resistance to low pH. Interestingly, the alternative sigma factor RpoS, which was induced during the stationary phase, affected the production of C19 CFA but not C17 CFA. Western blotting analysis showed that the increase in expression of CFA synthase at early stationary phase was due to the alternative sigma factor RpoS.
Abbreviations: ATR, acid tolerance response; CFA, cyclopropane fatty acid; UFA, unsaturated fatty acid
The GenBank/EMBL/DDBJ accession number for the sequence of the cfa gene determined in this study is AF417203.
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