HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Blanchin-Roland, S. Articles by Gaillardin, C. Search for Related Content PubMed PubMed Citation Articles by Blanchin-Roland, S. Articles by Gaillardin, C. Agricola Articles by Blanchin-Roland, S. Articles by Gaillardin, C.

ESCRT-I components of the endocytic machinery are required for Rim101-dependent ambient pH regulation in the yeast Yarrowia lipolytica

Microbiologie et Génétique Moléculaire, Institut National Agronomique Paris-Grignon, Institut National de la Recherche Agronomique UMR1238, Centre National de la Recherche Scientifique UMR2585, 78850 Thiverval-Grignon, France

Correspondence
Sylvie Blanchin-Roland
blanchin{at}grignon.inra.fr

Ambient pH signalling involves a cascade of conserved Rim or Pal products in ascomycetous yeasts or filamentous fungi, respectively. Insertional mutagenesis in the yeast Yarrowia lipolytica identified two components of the endosome-associated ESCRT-I complex involved in multivesicular body (MVB) vesicle formation, YlVps28p and YlVps23p. They were shown to be required at alkaline pH, like Rim factors, for transcriptional activation of alkaline-induced genes and repression of acid-induced genes. The constitutively active YlRIM101-1119 allele, which suppresses the pH-signalling defects of Ylrim mutations, also suppresses Ylvps defects in pH response, but not in endocytosis. The contribution of the ESCRT-III component Snf7p could not be assessed due to the essential nature of this component in Y. lipolytica. Unlike Rim factors, YlVps4p, a component of the MVB pathway acting downstream from ESCRT complexes, seems not to be required for the alkaline response. In Y. lipolytica, all vps mutations including those affecting YlVPS4, affected growth at acidic pH, a feature not exhibited by Ylrim mutations. These results suggest that Rim and Vps pathways cooperate in ambient pH signalling and that this relation is conserved across the full range of hemiascomycetous yeasts.

The GenBank/EMBL/DDBJ accession number for the sequence reported in this paper is AJ509167.

This article has been cited by other articles:

				S. Mansour, J. Bailly, S. Landaud, C. Monnet, A. S. Sarthou, M. Cocaign-Bousquet, S. Leroy, F. Irlinger, and P. Bonnarme Investigation of Associations of Yarrowia lipolytica, Staphylococcus xylosus, and Lactococcus lactis in Culture as a First Step in Microbial Interaction Analysis Appl. Envir. Microbiol., October 15, 2009; 75(20): 6422 - 6430. [Abstract] [Full Text] [PDF]

				O. Rodriguez-Galan, A. Galindo, A. Hervas-Aguilar, H. N. Arst Jr., and M. A. Penalva Physiological Involvement in pH Signaling of Vps24-mediated Recruitment of Aspergillus PalB Cysteine Protease to ESCRT-III J. Biol. Chem., February 13, 2009; 284(7): 4404 - 4412. [Abstract] [Full Text] [PDF]

				S. Mansour, J. M. Beckerich, and P. Bonnarme Lactate and Amino Acid Catabolism in the Cheese-Ripening Yeast Yarrowia lipolytica Appl. Envir. Microbiol., November 1, 2008; 74(21): 6505 - 6512. [Abstract] [Full Text] [PDF]

				S. Blanchin-Roland, G. Da Costa, and C. Gaillardin Ambient pH signalling in the yeast Yarrowia lipolytica involves YlRim23p/PalC, which interacts with Snf7p/Vps32p, but does not require the long C terminus of YlRim9p/PalI Microbiology, June 1, 2008; 154(6): 1668 - 1676. [Abstract] [Full Text] [PDF]

				M. Cornet, C. Gaillardin, and M. L. Richard Deletions of the Endocytic Components VPS28 and VPS32 in Candida albicans Lead to Echinocandin and Azole Hypersensitivity. Antimicrob. Agents Chemother., October 1, 2006; 50(10): 3492 - 3495. [Abstract] [Full Text] [PDF]

				J. H. Boysen and A. P. Mitchell Control of Bro1-Domain Protein Rim20 Localization by External pH, ESCRT Machinery, and the Saccharomyces cerevisiae Rim101 Pathway Mol. Biol. Cell, March 1, 2006; 17(3): 1344 - 1353. [Abstract] [Full Text] [PDF]