Microbiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Microbiology 151 (2005), 439-446; DOI  10.1099/mic.0.27411-0
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Golic, N.
Right arrow Articles by van Kranenburg, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Golic, N.
Right arrow Articles by van Kranenburg, R.
Agricola
Right arrow Articles by Golic, N.
Right arrow Articles by van Kranenburg, R.
Microbiology 151 (2005), 439-446; DOI  10.1099/mic.0.27411-0
© 2005 Society for General Microbiology

Molecular characterization of the CmbR activator-binding site in the metC–cysK promoter region in Lactococcus lactis

Natasa Golic1,2,3, Martijn Schliekelmann1,2, María Fernández1,2,{ddagger}, Michiel Kleerebezem1,2 and Richard van Kranenburg1,2,{dagger}

1 Wageningen Centre for Food Sciences, Wageningen, The Netherlands
2 Flavour, Nutrition and Ingredients Department, NIZO Food Research, PO Box 20, 6710 BA Ede, The Netherlands
3 Institute of Molecular Genetics and Genetic Engineering, Belgrade, Yugoslavia

Correspondence
Michiel Kleerebezem
michiel.kleerebezem{at}nizo.nl

The metC–cysK operon involved in sulphur metabolism in Lactococcus lactis is positively regulated by the LysR-type protein CmbR. Transcription from the metC promoter is activated when concentrations of methionine and cysteine in the growth medium are low. The metC promoter region contains two direct and three inverted repeats. Deletion analysis indicated that direct repeat 2 (DR2) is required for activation of the metC promoter by CmbR. Gel mobility shift assays confirmed that CmbR binds to a 407 bp DNA fragment containing the metC promoter. This binding was stimulated by O-acetyl-L-serine. Competition experiments with deletion variants of the metC promoter showed that CmbR binding only occurred with fragments containing an intact DR2, confirming that DR2 is the CmbR binding site within the metC promoter.

Abbreviations: CFE, cell-free extract; DR, direct repeat; LTTR, LysR-type transcriptional regulator; OAS, O-acetyl-L-serine

{dagger}Present address: Purac Biochem, Gorinchem, The Netherlands.

{ddagger}Present address: Instituto de Productos Lácteos de Asturias (CSIC) C/Infiesto s/n Villaviciosa, Asturias, Spain.




This article has been cited by other articles:


Home page
 Appl. Environ. Microbiol.Home page
M. Liu, R. J. Siezen, and A. Nauta
In Silico Prediction of Horizontal Gene Transfer Events in Lactobacillus bulgaricus and Streptococcus thermophilus Reveals Protocooperation in Yogurt Manufacturing
Appl. Envir. Microbiol., June 15, 2009; 75(12): 4120 - 4129.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
M. Liu, A. Nauta, C. Francke, and R. J. Siezen
Comparative Genomics of Enzymes in Flavor-Forming Pathways from Amino Acids in Lactic Acid Bacteria
Appl. Envir. Microbiol., August 1, 2008; 74(15): 4590 - 4600.
[Full Text] [PDF]

Home page
 J. Bacteriol.Home page
B. Sperandio, C. Gautier, S. McGovern, D. S. Ehrlich, P. Renault, I. Martin-Verstraete, and E. Guedon
Control of Methionine Synthesis and Uptake by MetR and Homocysteine in Streptococcus mutans
J. Bacteriol., October 1, 2007; 189(19): 7032 - 7044.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
C. D. den Hengst, M. Groeneveld, O. P. Kuipers, and J. Kok
Identification and Functional Characterization of the Lactococcus lactis CodY-Regulated Branched-Chain Amino Acid Permease BcaP (CtrA)
J. Bacteriol., May 1, 2006; 188(9): 3280 - 3289.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
P. Burguiere, J. Fert, I. Guillouard, S. Auger, A. Danchin, and I. Martin-Verstraete
Regulation of the Bacillus subtilis ytmI Operon, Involved in Sulfur Metabolism
J. Bacteriol., September 1, 2005; 187(17): 6019 - 6030.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2005 Society for General Microbiology.