HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (Papers in Press[PDF]) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Google Scholar Articles by Lee, Y. J. Articles by Nihira, T. PubMed PubMed Citation Articles by Lee, Y. J. Articles by Nihira, T. Agricola Articles by Lee, Y. J. Articles by Nihira, T.

Null mutation analysis of an afsA-family gene, barX, that is involved in biosynthesis of the -butyrolactone autoregulator in Streptomyces virginiae

Osaka University

Virginiae butanolide (VB) is a -butyrolactone autoregulator triggering production of the streptogramin antibiotic virginiamycin in Streptomyces virginiae. Our previous studies suggested that the barX gene, an afsA-family gene, is likely to participate in the regulatory pathway for the production of VB, rather than in the biosynthetic pathway of VB itself, in contrast to the function of other afsA-family genes. Mutation analysis now shows that BarX at least plays an enzymatic role in the VB biosynthetic pathway. Heterologous expression of the afsA gene from Streptomyces griseus into the barX mutant partially restored the deficiency of virginiamycin production, suggesting that afsA-family genes have a common ability to synthesize the -butyrolactone autoregulators. Taken together with previous works relating the function of an afsA-family gene, these results supported an idea that streptomycetes has two biosynthetic pathways for the -butyrolactone autoregulators.

¹ E-mail: nihira{at}icb.osaka-u.ac.jp