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Published online ahead of print on 5 November 2009 as doi:10.1099/mic.0.032730-0
Microbiology (2009), DOI 10.1099/mic.0.032730-0
© 2009 Society for General Microbiology
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Microbiology 0 (2009), mic.0.032730; DOI  10.1099/mic.0.032730-0
© 2009 Society for General Microbiology

Quorum Sensing Regulated Virulence factors in Pseudomonas aeruginosa are toxic to Lucilia sericata maggots

Anders Schou Andersen1,4, Bo Jørgensen2, Thomas Bjarnsholt3, Helle Johansen3, Tonny Karlsmark2, Michael Givskov3 and Karen A. Krogfelt1

1 Statens Serum Institut;
2 Copenhagen Wound Healing Centre, Bispebjerg Hospital;
3 University of Copenhagen

Maggot debridement therapy (MDT) is widely used for debridement of chronic infected wounds, however for wounds harbouring specific bacteria limited effect or failure of the treatment have been described. Here we study the survival of Lucilia sericata maggots encountering Pseudomonas aeruginosa (PAO1) in a simple assay with emphasis on the quorum sensing (QS) regulated virulence. The maggots were challenged with GFP tagged P.aeruginosa WT PAO1 and GFP tagged P. aeruginosa {Delta}lasR rhlR ({Delta}RR) QS deficient mutants in different concentrations. Maggots were killed in the presence of WT PAO1 whereas the challenge with the QS mutants showed a survival reduction of (approx 25%) compared to negative controls. Furthermore, bacterial intake by the maggots was reduced in the presence of PAO1, but not by PAO1 ({Delta}RR) mutant. Maggot excretion/secretions (ES) were assayed for the presence of QS inhibitors and only high doses of ES showed inhibition of QS in P. aeruginosa. Thus P. aeruginosa was shown to be toxic to L. sericata maggots. This coupled to the preferential feeding by the maggots and reduced ingestion of P. aeruginosacould explain the MDT failure in wounds colonised by P. aeruginosa. Wounds heavily colonised with P. aeruginosa should be a counter indication for MDT unless used in combination with a pre-treatment with other topical therapeutics targeting P. aeruginosa.

4 E-mail: aea{at}ssi.dk






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