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A broad host range flagellum-dependent phage mediates high-efficiency generalized transduction in, and between, Serratia and Pantoea

¹ University of Cambridge;
² Wellcome Trust Sanger Institute;
³ Leatherhead Food Research

A phage (OT8) isolated on Serratia sp. ATCC 39006 was shown to be flagellum-dependent and to mediate generalized transduction with high efficiency (up to 10^-4 transductants per p.f.u.). OT8 has a broad host range because it also infects a strain of Pantoea agglomerans isolated from the rhizosphere. Transduction of plasmid-borne antibiotic resistance between these two bacterial genera was demonstrated, consistent with purported ecological roles of phages in dissemination of genes between bacterial genera. Serratia sp. ATCC 39006 and Pantoea agglomerans produce a number of interesting secondary metabolites with potential applications in cancer therapy and biocontrol of fungal infections. OT8 has utility as a powerful functional genomics tool in these bacteria.

⁴ E-mail: g.p.c.salmond{at}bioc.cam.ac.uk