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Published online ahead of print on 22 October 2009 as doi:10.1099/mic.0.033290-0
Microbiology (2009), DOI 10.1099/mic.0.033290-0
© 2009 Society for General Microbiology
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Microbiology 0 (2009), mic.0.033290; DOI  10.1099/mic.0.033290-0
© 2009 Society for General Microbiology

Genetic determinants of Pseudomonas aeruginosa biofilm establishment

Mathias Müsken1, stefano DiFiore2, Andreas Dötsch3, Rainer Fischer4 and Susanne Haussler5,6

1 Helmholtz Center for Infection Research;
2 Institute for Molecular Biotechnology, RWTH Aachen University, Worringerweg 1, D-52704 Aachen, Germa;
3 Helmholtz Centre for Infection Research;
4 Fraunhofer Institute for Molecular Biology and Applied Ecology, Forckenbeck Str.6, D-52704 Aachen;
5 Helmholtz institute

The establishment of bacterial biofilms on surfaces is a complex process that requires various factors for each consecutive developmental step. Here we report the screen of the comprehensive Harvard Pseudomonas aeruginosa PA14 mutant library for mutants exhibiting an altered biofilm phenotype. We analyzed the capability of all mutants to form biofilms at the bottom of a 96-well plate by the use of an automated confocal laser scanning microscope and uncovered 394 and 285 genetic determinants of reduced and enhanced biofilm production, respectively. Overall, 67% of the identified mutants were affected within genes encoding for hypothetical proteins indicating that novel developmental pathways are likely to be dissected in the future. Nevertheless, a common theme that emerged from the analysis of the genes with a predicted function is that the establishment of a biofilm requires regulatory components that are involved in survival under microaerophilic growth conditions, arginine metabolism, alkyl-quinolone signaling, pH homeostasis and the DNA repair system.

6 E-mail: susanne.haeussler{at}helmholtz-hzi.de






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