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Cellulose modulates biofilm formation by counteracting curli-mediated colonization of solid surfaces in Escherichia coli, by L. Gualdi, L. Tagliabue, S. Bertagnoli, T. Ieranò, C. De Castro and P. Landini

Microbiology vol. 154, part 7, pp. 2017 - 2024

Fig. S1. Phenotypes of strains MG1655 (WT), PHL856 (csgA) and LG26 (ΔbcsA) on Congo Red-supplemented agar medium (CR medium), either at 30°C (upper panel) or at 37°C (lower panel). Strains were transformed either with the pT7-7 control vector or with the pT7CsgD plasmid as indicated. [PDF] (29 kb)

Fig. S2. SDS-PAGE of formic-acid-treated membrane fractions of MG1655 (WT); PHL856 (csgA::kan); LG26 (Δbcs::kan) transformed either with the pT7-7 or with the pT7CsgD plasmid, grown at 30°C. The band indicated by the arrow has an apparent molecular mass of 17 kDa, consistent with the molecular mass of CsgA, the curli major subunit. [PDF] (36 kb)







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